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PURPOSE: Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) has been deemed an oncogene in many human cancers. However, the underlying mechanism of NEAT1 in nasopharyngeal carcinoma (NPC) progression remains largely unclear. MATERIALS AND METHODS: Quantitative real-time PCR assay was performed to assess the expression of NEAT1 and miR-34a-5p in NPC tissues and cells. Western blot analysis was used to observe cell epithelial to mesenchymal transition (EMT) and the activation of Wnt/β-catenin signaling in 5-8F cells. MiRNA directly interacting with NEAT1 were verified by dual-luciferase reporter assay and RNA immunoprecipitation. Cell proliferation ability was determined by CCK-8 assay, and cell migration and invasion capacities were assessed by transwell assays. An animal model was used to investigate the regulatory effect of NEAT1 on tumor growth in vivo. RESULTS: Our data revealed that NEAT1 is upregulated, while miR-34a-5p is downregulated in NPC tissues and cell lines. NEAT1 knockdown repressed tumor growth in vitro and in vivo. Additionally, we discovered that NEAT1 directly binds to miR-34a-5p and suppresses miR-34a-5p expression. Moreover, NEAT1 knockdown exerted suppression effects on cell proliferation, migration, invasion, and EMT by miR-34a-5p. NEAT1 knockdown blocked Wnt/β-catenin signaling via miR-34a-5p. CONCLUSION: Our study demonstrated that NEAT1 targets miR-34a-5p at least partly to drive NPC progression by regulating Wnt/β-catenin signaling, suggesting a potential therapeutic target for NPC.
Subject(s)
Humans , Blotting, Western , Cell Line , Cell Movement , Cell Proliferation , Immunoprecipitation , In Vitro Techniques , MicroRNAs , Models, Animal , Oncogenes , Real-Time Polymerase Chain Reaction , RNA , RNA, Long Noncoding , SincalideABSTRACT
Objective@#To explore factors associated with fatigue in employees working in Internet companies.@*Methods@#A cross-sectional study was conducted among 3603 employees from 35 internet companies. A self-conducted questionnaire was used to assess employees’ fatigue and related factors.@*Results@#The scores of body fatigue, mental fatigue and total fatigue were (4.53±2.56) , (2.37±1.64) , and (6.90±3.55) respectively. The body fatigue is positively correlated with job burnout and musculoskeletal disorders (r=0.426, 0.485) ; the mental fatigue is positively correlated with job burnout (r=0.429) . JDC and ERI occupational stress, burnout and high level of musculoskeletal disorders increased the risk of body fatigue of which odds ratios are 1.58, 1.72, 4.08 and 5.91; odds ratios for the risk of mental fatigue are 1.73, 1.37, 2.61 and 2.08. Sleep time over 7 hours reduces the risk of fatigue (P<0.05) with odds ratio of 0.61 and 0.62.@*Conclusion@#Employees of Internet companies is facing fatigue issues. To protect employee’s physical and mental health is highly important for employers to alleviate fatigue and improve work performance.
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Objective@#To investigate the association of occupational stress with job burnout and depression tendency in workers in Internet companies. @*Methods@#From July to November, 2016, the cross-sectional method was used to perform a questionnaire survey of 3603 workers in 35 Internet companies in Beijing, Shandong Province, and Zhejiang Province in China, and the association of occupational stress with job burnout and depression tendency was analyzed. @*Results@#Among these workers, 63.70% had occupational stress with job demand-control (JDC) and 34.60% had occupational stress with effort-reward imbalance (ERI) ; among the workers engaged in sales, 75.63% had occupational stress with JDC and 62.70% had occupational stress with ERI. Of all workers, 10.69% had job burnout, and among the workers engaged in sales, 22.12% had job burnout. Of all workers, 18.79% had the tendency of moderate-to-severe or severe depression, and among the workers engaged in sales, 46.13% had such tendency. Occupational stress with JDC increased the risk of job burnout and depression (odds ratio[OR]=3.52 and 1.85, P<0.05) , and occupational stress with ERI also increased the risk of job burnout and depression (OR=8.24 and 5.59, P<0.05) . In addition, irregular diet and insomnia were risk factors for job burnout; age ≥41 years, low income, sales position, working time spent on the screen ≥10 hours/day, insomnia, and poor self-evaluated health status were risk factors for depression tendency. @*Conclusion@#Occupational stress with JDC and ERI increases the risk of job burnout and depression tendency, and among the workers in Internet companies, the workers engaged in sales have the most severe occupational stress, job burnout, and depression tendency.
ABSTRACT
OBJECTIVE To establish an in vitro test method and to evaluate the genotoxicity of chemicals using primary cultured mouse hepatocytes and the changes in phosphorylated histone H2AX(γH2AX)expression levels to provide a more reliable marker of the identification of genotoxicity. METHODS Hepatocytes were isolated from BALB/c mice by an improved two-step collagenase diges?tion method and then cultured in sandwich configuration. The primary cultured hepatocytes were treat?ed with various concentrations of four known genotoxic agents bleomycin(BLM),benzo(a)pyrene〔B (a)p〕,styrene and styrene-7,8-oxide(SO)within the range of 40 μmol · L-1 and two non-genotoxic agents azathioprine(Aza)and ciclosporin A(CsA)at different time points within 24 h. The cytotoxicity induced by these toxicants was assessed by CCK-8 assay. Then,the changes in γH2AX expression levels in treated cells were determined by flow cytometry. RESULTS The four genotoxic agents could be detected and two non-genotoxic agents could not be detected by this method. The γH2AX expression level was the highest when hepatocytes were exposed to BLM and SO for 3 h,or B(a)p and styrene for 6 h(P<0.01). The production of γH2AX was 25.67,18.36,12.43 and 14.25 for the four types of genotoxic agents,respectively,and was approximately 19,13,9 and 11 times that of the vehicle control group(P<0.01)at the optimum time point and concentration. There was a significant positive corre?lation between the indicated concentrations of genotoxic chemicals and γH2AX expression levels(P<0.01). In addition,the production ofγH2AX indicated no marked increase in two non-genotoxic agents such as Aza and CsA in comparison with the control group. CONCLUSION This test method can effec?tively distinguish genotoxic agents from non-genotoxic agents,and direct genotoxic agents from indirect genotoxic agents in the absence of S9. γH2AX might be a reliable marker for the identification of the potential genotoxicity of chemicals.